Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- 14503-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14503-1-AP, RRID:AB_2218096
- Product name
- 14-3-3 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated 14-3-3 antibody (Cat. #14503-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles.
HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology.
Nedd4-2 haploinsufficiency in mice causes increased seizure susceptibility and impaired Kir4.1 ubiquitination.
14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation.
Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding.
Regulatory factor X5 promotes hepatocellular carcinoma progression by transactivating tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta and suppressing apoptosis.
Glucocorticoids and myosin5b loss of function induce heightened PKA signaling in addition to membrane traffic defects.
Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.
Huang B, Zhao Y, Zhou L, Gong T, Feng J, Han P, Qian J
Journal of inflammation research 2021;14:3489-3500
Journal of inflammation research 2021;14:3489-3500
HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology.
Tanaka H, Kondo K, Fujita K, Homma H, Tagawa K, Jin X, Jin M, Yoshioka Y, Takayama S, Masuda H, Tokuyama R, Nakazaki Y, Saito T, Saido T, Murayama S, Ikura T, Ito N, Yamamori Y, Tomii K, Bianchi ME, Okazawa H
Communications biology 2021 Oct 11;4(1):1175
Communications biology 2021 Oct 11;4(1):1175
Nedd4-2 haploinsufficiency in mice causes increased seizure susceptibility and impaired Kir4.1 ubiquitination.
Liu X, Zhang H, Zhang B, Tu J, Li X, Zhao Y
Biochimica et biophysica acta. Molecular basis of disease 2021 Jun 1;1867(6):166128
Biochimica et biophysica acta. Molecular basis of disease 2021 Jun 1;1867(6):166128
14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation.
Kundu A, Shelar S, Ghosh AP, Ballestas M, Kirkman R, Nam H, Brinkley GJ, Karki S, Mobley JA, Bae S, Varambally S, Sudarshan S
The Journal of biological chemistry 2020 Feb 7;295(6):1754-1766
The Journal of biological chemistry 2020 Feb 7;295(6):1754-1766
Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding.
Norkett R, Del Castillo U, Lu W, Gelfand VI
eLife 2020 Feb 5;9
eLife 2020 Feb 5;9
Regulatory factor X5 promotes hepatocellular carcinoma progression by transactivating tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta and suppressing apoptosis.
Chen DB, Zhao YJ, Wang XY, Liao WJ, Chen P, Deng KJ, Cong X, Fei R, Wu X, Shao QX, Wei L, Xie XW, Chen HS
Chinese medical journal 2019 Jul 5;132(13):1572-1581
Chinese medical journal 2019 Jul 5;132(13):1572-1581
Glucocorticoids and myosin5b loss of function induce heightened PKA signaling in addition to membrane traffic defects.
Forteza R, Ahsan MK, Cartón-García F, Arango D, Ameen NA, Salas PJ
Molecular biology of the cell 2019 Dec 15;30(26):3076-3089
Molecular biology of the cell 2019 Dec 15;30(26):3076-3089
Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.
Bai C, Tang S, Bai C, Chen X
Amino acids 2014 Apr;46(4):841-52
Amino acids 2014 Apr;46(4):841-52
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP(14-3-3 antibody) at dilution of 1:400
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-14-3-3 (IP:14503-1-AP, 5ug; Detection:14503-1-AP 1:500) with mouse lung tissue lysate 4000ug.
- Sample type
- tissue