Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX102199 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102199, RRID:AB_1952636
- Product name
- 14-3-3 theta antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse brain 12% SDS PAGE GTX102199 diluted at 1:30000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
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- Experimental details
- Sample(30 ?g whole cell lysate) A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: HepG2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908)12% SDS PAGEGTX102199 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody (GTX102199) diluted at 1:1000 and competitor's antibody (SC-732) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 theta antibody detects 14-3-3 theta protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 14-3-3 theta antibody (GTX102199) diluted at 1:500.