GTX112026

antibody from GeneTex

Targeting: YWHAQ 14-3-3, HS1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX112026

Provider

GeneTex

Proper citation

GeneTex Cat#GTX112026, RRID:AB_1952634

Product name

14-3-3 theta antibody [N1C3-2]

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE GTX112026 diluted at 1:10000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 (GTX27900) 12% SDS PAGE GTX112026 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 theta antibody [N1C3-2] (GTX112026) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody [N1C3-2] (GTX112026) diluted at 1:1000 and competitor's antibody (sc-732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

14-3-3 theta antibody [N1C3-2] detects 14-3-3 theta protein at cytoplasm and nucleus on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. 14-3-3 theta antibody [N1C3-2] (GTX112026) dilution: 1:500.