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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PPS042 - Provider product page
- Provider
- R&D Systems
- Product name
- Phospho-14-3-3 (S58) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. This antibody is specific for the 29 kDa 14-3-3 phosphorylated at S58 in Western blots of rat brain stem lysates.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Sheep, Simian, Xenopus, Zebrafish
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Phospho-14-3-3 (S58) by Western Blot. Western blot of rat brainstem lysate showing specific immunolabeling of the approximately 29 kDa 14-3-3 protein phosphorylated at S58 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phosphopeptide Block) but not by the corresponding dephosphopeptide (not shown).
