PAB20399

antibody from Abnova Corporation

Targeting: YWHAQ 14-3-3, HS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20399

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20399, RRID:AB_10970429

Product name

YWHAQ polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant YWHAQ.

Antigen sequence

ELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKT
DTSDKKLQLIKDYREKVESELRSICTTVLELLDKY
LIANATNPESKVFYLKMKGDYFRY


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with YWHAQ polyclonal antibody (Cat # PAB20399).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with YWHAQ polyclonal antibody (Cat # PAB20399).Lane 1 : NIH/3T3Lane 2 : NBT-II

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with YWHAQ polyclonal antibody (Cat # PAB20399) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, cytoplasm, centrosome.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with YWHAQ polyclonal antibody (Cat # PAB20399) shows strong cytoplasmic positivity in neuronal cells and glial cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)