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- Product number
- NBP1-21301 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-21301, RRID:AB_1659853
- Product name
- Mouse Monoclonal 14-3-3 tau/theta Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Differential Subcellular Distribution and Translocation of Seven 14-3-3 Isoforms in Response to EGF and During the Cell Cycle.
Abdrabou A, Brandwein D, Wang Z
International journal of molecular sciences 2020 Jan 2;21(1)
International journal of molecular sciences 2020 Jan 2;21(1)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - 14-3-3 tau/theta Antibody (1A1) Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 theta (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - The recombinant proteins (50ng) were resolved by SDSPAGE, transferred to PDVF membrane and probed with antihuman 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human YWHAZ Lane 2. : Recombinant Human YWHAB Lane 3. : Recombinant Human YWHAE Lane 4. : Recombinant Human YWHAH Lane 5. : Recombinant Human YWHAG Lane 6. : Recombinant Human SFN Lane 7. : Recombinant Human YWHAQ (14-3-3 tau/theta)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MDA-MB-21 cell lysate Lane 2. : HeLa cell lysate Lane 3. : A431 cell lysate Lane 4. : NIH3T3 cell lysate Lane 5. : 293T cell lysate Lane 6. : HepG2 cell lysate
