PA1-4647

antibody from Invitrogen Antibodies

Targeting: YWHAQ 14-3-3, HS1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-4647

Provider

Invitrogen Antibodies

Product name

Anti-14-3-3

Antibody type

Polyclonal

Antigen

Peptide corresponding to amino acid residues from the C-terminal region of 14-3-3 protein.

Reactivity

Rat

Host

Rabbit

Vial size

100 ul

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of YWHAB was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with 14-3-3 beta Polyclonal Antibody (Product # PA1-4647) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Membrane, Cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.