PA5-27641

antibody from Invitrogen Antibodies

Targeting: YWHAQ 14-3-3, HS1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27641

Provider

Invitrogen Antibodies

Product name

14-3-3 theta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, H1299, HeLaS3, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (80%), Rabbit (99%), Chicken (99%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.84 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using 14-3-3 theta Polyclonal Antibody (Product # PA5-27641). Sample (30 µg whole cell lysate). Lane A: 293T. B: A431. C: H1299. D: HeLa S3. E: HepG2. F: MOLT4. G: Raji. 12% SDS PAGE.14-3-3 theta Polyclonal Antibody (Product # PA5-27641) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using 14-3-3 theta Polyclonal Antibody (Product # PA5-27641). Sample (30 µg whole cell lysate). Lane A: 293T. B: A431. C: H1299. D: HeLa S3. E: HepG2. F: MOLT4. G: Raji. 12% SDS PAGE.14-3-3 theta Polyclonal Antibody (Product # PA5-27641) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using 14-3-3 theta Polyclonal Antibody (Product # PA5-27641). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. 14-3-3 theta Polyclonal Antibody (Product # PA5-27641) diluted at 1:30,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of 14-3-3 theta was performed in paraffin-embedded human breast carcinoma tissue using 14-3-3 theta Polyclonal Antibody (Product # PA5-27641) at a dilution of 1:500.