PA5-29421

antibody from Invitrogen Antibodies

Targeting: YWHAQ 14-3-3, HS1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29421

Provider

Invitrogen Antibodies

Product name

14-3-3 theta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain.

Concentration

0.98 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of 14-3-3 theta using 30 µg of A) HeLa and B) HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a 14-3-3 theta polyclonal antibody (Product # PA5-29421) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of 14-3-3-theta was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a 14-3-3 theta Polyclonal Antibody (Product # PA5-29421) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of 14-3-3-theta was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a 14-3-3 theta Polyclonal Antibody (Product # PA5-29421) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using 14-3-3 theta Polyclonal Antibody (Product # PA5-29421). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. 14-3-3 theta Polyclonal Antibody (Product # PA5-29421) diluted at 1:10,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

14-3-3 theta Polyclonal Antibody detects 14-3-3 theta protein at cytoplasm and nucleus on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft.14-3-3 theta Polyclonal Antibody (Product # PA5-29421) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.