AF4425

antibody from R&D Systems

Targeting: YWHAQ 14-3-3, HS1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4425

Provider

R&D Systems

Product name

Human/Mouse/Rat 14-3-3 theta Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat 14-3-3 theta in Western blots. Does not cross-react with other recombinant human 14-3-3 isoforms.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P27348


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/ Rat 14-3-3 theta by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromo-cytoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3 theta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4425) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14-3-3 theta was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.