Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- PAB6168 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6168, RRID:AB_1572704
- Product name
- ARPC2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of ARPC2.
- Antigen sequence
C-EMKTITGKTFSSR
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ
The Journal of cell biology 1997 Jul 28;138(2):375-84
The Journal of cell biology 1997 Jul 28;138(2):375-84
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ARPC2 polyclonal antibody (Cat # PAB6168) staining (0.2 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ARPC2 polyclonal antibody (Cat # PAB6168) (3.8 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)