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Western blot
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- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-1037 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1037, RRID:AB_2227542
- Product name
- Goat Polyclonal ARPC2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Flat clathrin lattices are dynamic actin-controlled hubs for clathrin-mediated endocytosis and signalling of specific receptors.
Initiation of lamellipodia and ruffles involves cooperation between mDia1 and the Arp2/3 complex.
Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1.
The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
Leyton-Puig D, Isogai T, Argenzio E, van den Broek B, Klarenbeek J, Janssen H, Jalink K, Innocenti M
Nature communications 2017 Jul 13;8:16068
Nature communications 2017 Jul 13;8:16068
Initiation of lamellipodia and ruffles involves cooperation between mDia1 and the Arp2/3 complex.
Isogai T, van der Kammen R, Leyton-Puig D, Kedziora KM, Jalink K, Innocenti M
Journal of cell science 2015 Oct 15;128(20):3796-810
Journal of cell science 2015 Oct 15;128(20):3796-810
Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1.
Sakata D, Taniguchi H, Yasuda S, Adachi-Morishima A, Hamazaki Y, Nakayama R, Miki T, Minato N, Narumiya S
The Journal of experimental medicine 2007 Sep 3;204(9):2031-8
The Journal of experimental medicine 2007 Sep 3;204(9):2031-8
The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ
The Journal of cell biology 1997 Jul 28;138(2):375-84
The Journal of cell biology 1997 Jul 28;138(2):375-84
The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ
The Journal of cell biology 1997 Jul 28;138(2):375-84
The Journal of cell biology 1997 Jul 28;138(2):375-84
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARPC2 Antibody [NB100-1037] - Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARPC2 Antibody [NB100-1037] - Staining of CACO-2 with antibody at 0.5 ug/mL (A), HeLa (B) and Jurkat (C) lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARPC2 Antibody [NB100-1037] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 3.8 ug/mL.
