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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab089679 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant ARPC2 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human ARPC2 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.Application¥ WB: 1/500-1/1000¥ IHC: 1/50-1/100¥ IF/ICC: 1/50-1/200Protein FunctionFunctions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 282-300- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,6 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ARPC2 Antibody (Ab089679) - Western Blot All lanes: Recombinant ARPC2 Antibody (Ab089679) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 33 kDa Exposure time: 2.5 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ARPC2 Antibody (Ab089679) - IF/ICC Immunocytochemistry analysis of ARPC2 (green) in HeLa cells using Recombinant ARPC2 Antibody (Ab089679), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ARPC2 Antibody (Ab089679) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant ARPC2 Antibody (Ab089679). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
