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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Other assay [2]
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- Product number
- PA5-22005 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ETFB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (92%), Rat (92%), Xenopus laevis (80%), Pig (95%), Rhesus Monkey (98%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.91 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Role of METTL20 in regulating β-oxidation and heat production in mice under fasting or ketogenic conditions.
Shimazu T, Furuse T, Balan S, Yamada I, Okuno S, Iwanari H, Suzuki T, Hamakubo T, Dohmae N, Yoshikawa T, Wakana S, Shinkai Y
Scientific reports 2018 Jan 19;8(1):1179
Scientific reports 2018 Jan 19;8(1):1179
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ETFB Polyclonal Antibody (Product # PA5-22005). Sample (30 µg of whole cell lysate). A: MOLT4. 12% SDS PAGE. ETFB Polyclonal Antibody (Product # PA5-22005) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ETFB Polyclonal Antibody (Product # PA5-22005). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. ETFB Polyclonal Antibody (Product # PA5-22005) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Beta-ETF in paraformaldehyde-fixed HeLa cells using a Beta-ETF polyclonal antibody (Product # PA5-22005) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-ETF antibody (Product # PA5-22005) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
