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Learn500-P03-100UG
antibody from Invitrogen Antibodies
Targeting: PPBP
b-TG1, Beta-TG, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, NAP-2-L1, PBP, SCYB7, TGB, TGB1, THBGB1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- ELISA [1]
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- Product number
- 500-P03-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPBP Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NAP-2 (CXCL7). Anti-Human NAP-2 (CXCL7)-specific antibody was purified by affinity chromatography employing an immobilized Human NAP-2 matrix. Sandwich ELISA: To detect Human NAP-2 (CXCL7) by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NAP-2 (CXCL7) (500-P03BT) as a detection antibody, allows the detection of at least 2000- 4000 pg/mL of Recombinant Human NAP-2 (CXCL7). Western Blot: To detect Human NAP-2 (CXCL7) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NAP-2 (CXCL7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Serglycin proteoglycan deletion induces defects in platelet aggregation and thrombus formation in mice.
Monocyte-derived CXCL7 peptides in the marrow microenvironment.
Requirement of VPS33B, a member of the Sec1/Munc18 protein family, in megakaryocyte and platelet alpha-granule biogenesis.
Woulfe DS, Lilliendahl JK, August S, Rauova L, Kowalska MA, Abrink M, Pejler G, White JG, Schick BP
Blood 2008 Apr 1;111(7):3458-67
Blood 2008 Apr 1;111(7):3458-67
Monocyte-derived CXCL7 peptides in the marrow microenvironment.
Pillai MM, Iwata M, Awaya N, Graf L, Torok-Storb B
Blood 2006 May 1;107(9):3520-6
Blood 2006 May 1;107(9):3520-6
Requirement of VPS33B, a member of the Sec1/Munc18 protein family, in megakaryocyte and platelet alpha-granule biogenesis.
Lo B, Li L, Gissen P, Christensen H, McKiernan PJ, Ye C, Abdelhaleem M, Hayes JA, Williams MD, Chitayat D, Kahr WH
Blood 2005 Dec 15;106(13):4159-66
Blood 2005 Dec 15;106(13):4159-66
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human NAP-2 (CXCL7) by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of PPBP Polyclonal Antibody (Product # 500-P03-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech PPBP Polyclonal Antibody, Biotin (Product # 500-P03BT-1MG) as a detection antibody, allows the detection of at least 2000- 4000 pg/mL of Recombinant Human NAP-2 (CXCL7).
