Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- IC36081G - Provider product page
- Provider
- R&D Systems
- Product name
- Human BLIMP1/PRDM1 Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BLIMP1/PRDM1 in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Antigen sequence
O75626
- Isotype
- IgG
- Antibody clone number
- 646702
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients.
Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis.
MAIT cells are licensed through granzyme exchange to kill bacterially sensitized targets.
Bankó Z, Pozsgay J, Szili D, Tóth M, Gáti T, Nagy G, Rojkovich B, Sármay G
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 15;198(4):1512-1520
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 15;198(4):1512-1520
Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis.
Rao DA, Gurish MF, Marshall JL, Slowikowski K, Fonseka CY, Liu Y, Donlin LT, Henderson LA, Wei K, Mizoguchi F, Teslovich NC, Weinblatt ME, Massarotti EM, Coblyn JS, Helfgott SM, Lee YC, Todd DJ, Bykerk VP, Goodman SM, Pernis AB, Ivashkiv LB, Karlson EW, Nigrovic PA, Filer A, Buckley CD, Lederer JA, Raychaudhuri S, Brenner MB
Nature 2017 Feb 1;542(7639):110-114
Nature 2017 Feb 1;542(7639):110-114
MAIT cells are licensed through granzyme exchange to kill bacterially sensitized targets.
Kurioka A, Ussher JE, Cosgrove C, Clough C, Fergusson JR, Smith K, Kang YH, Walker LJ, Hansen TH, Willberg CB, Klenerman P
Mucosal immunology 2015 Mar;8(2):429-40
Mucosal immunology 2015 Mar;8(2):429-40
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC36081G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of BLIMP1/PRDM1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD19+ CD3- cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC36081G) and Mouse Anti-Human CD38 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.