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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AM1869a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1869a, RRID:AB_10814028
- Product name
- RET Antibody (Ascites)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 100 µl
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- RET (Cat. #AM1869a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RET antibody detected the RET protein (arrow).
- Primary Ab dilution
- 1:500~16000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of RET Antibody (Ascites)(Cat#AM1869a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor? 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- RET Monoclonal( Ascites) (Cat. #AM1869a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RET Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100
