PAB3381

antibody from Abnova Corporation

Targeting: RET CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3381

Provider

Abnova Corporation

Proper citation

Product name

RET polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of RET.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Intronic single nucleotide polymorphisms in the RET protooncogene are associated with a subset of apparently sporadic pheochromocytoma and may modulate age of onset.

McWhinney SR, Boru G, Binkley PK, Peczkowska M, Januszewicz AA, Neumann HP, Eng C
The Journal of clinical endocrinology and metabolism 2003 Oct;88(10):4911-6

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The tyrosine phosphatase Shp-2 mediates intracellular signaling initiated by Ret mutants.

D'Alessio A, Califano D, Incoronato M, Santelli G, Florio T, Schettini G, Carlomagno MS, Cerchia L, de Franciscis V
Endocrinology 2003 Oct;144(10):4298-305

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A novel germ-line point mutation in RET exon 8 (Gly(533)Cys) in a large kindred with familial medullary thyroid carcinoma.

Da Silva AM, Maciel RM, Da Silva MR, Toledo SR, De Carvalho MB, Cerutti JM
The Journal of clinical endocrinology and metabolism 2003 Nov;88(11):5438-43

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of MCF-7 cell lysate (35 ug/lane) with RET polyclonal antibody (Cat # PAB3381).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human brainreacted with RET polyclonal antibody (Cat # PAB3381), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Flow cytometric analysis of MCF-7 cells using RET polyclonal antibody (Cat # PAB3381)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Validation comment

Flow Cytometry