PAB16250

antibody from Abnova Corporation

Targeting: P2RX7 MGC20089, P2X7

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB16250

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB16250, RRID:AB_10696397

Product name

P2RX7 polyclonal antibody

Antibody type

Polyclonal

Description

Goat polyclonal antibody raised against synthetic peptide of P2RX7.

Antigen sequence

YETNKVTRIQSMNY-C


Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Activated microglia in ischemic stroke penumbra upregulate MCP-1 and CCR2 expression in response to lysophosphatidylcholine derived from adjacent neurons and astrocytes.

Inose Y, Kato Y, Kitagawa K, Uchiyama S, Shibata N
Neuropathology : official journal of the Japanese Society of Neuropathology 2015 Jun;35(3):209-23

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The human cathelicidin LL-37 modulates the activities of the P2X7 receptor in a structure-dependent manner.

Tomasinsig L, Pizzirani C, Skerlavaj B, Pellegatti P, Gulinelli S, Tossi A, Di Virgilio F, Zanetti M
The Journal of biological chemistry 2008 Nov 7;283(45):30471-81

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

P2RX7 polyclonal antibody (Cat # PAB16250) staining (0.3 ug/mL) of human brain (frontal cortex) lysate (35 ug protein in RIPA buffer) with (Lane 2) and without (Lane 1) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

P2RX7 polyclonal antibody (Cat # PAB16250) (3.8 ug/mL) staining of paraffin embedded human brain cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)