PA5-17024

antibody from Invitrogen Antibodies

Targeting: CDT1 DUP, RIS2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17024

Provider

Invitrogen Antibodies

Product name

CDT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CDT1 in extracts from Jurkat and Hela cells using CDT1 polyclonal antibody (Product # PA5-17024).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CDT1 Polyclonal Antibody (Product # PA5-17024) and a 60 kDa band corresponding to CDT1 was observed upon across the samples tested and was also increased upon double thymidine block followed by serum release in U-2 OS. Modified whole cell extracts (1% SDS) (30 µg lysate) of HT-29 (Lane 1), HeLa (Lane 2), U-2 OS (Lane 3) and U-2 OS treated with double thymidine block for 14 hrs followed by serum release for 9hrs (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).