720367

antibody from Invitrogen Antibodies

Targeting: FPR2 ALXR, FMLP-R-II, FMLPX, FPR2A, FPRH2, FPRL1, HM63, LXA4R

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

720367

Provider

Invitrogen Antibodies

Product name

FPR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells were transiently transfected with Formyl peptide receptor 1/FPR1 or Formyl peptide receptor 2/FPR2. After 48 h, cells were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-Formyl peptide receptor 2/FPR2 Rabbit Polyclonal Antibody (Product # 720367 at a concentration of 2 µg/mL. A 50 kDa corresponding to Formyl peptide receptor 2/FPR2 was observed.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sections of human kidney were dewaxed, microwaved in citric acid and incubated with Anti-Formyl peptide receptor 2/FPR2 Rabbit Polyclonal Antibody (Product # 720367 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining of blood vessels is completely neutralized by preincubation of the antibody with its immunizing peptide.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sections of human cerebral cortex were dewaxed, microwaved in citric acid and incubated withAnti-Formyl peptide receptor 2/FPR2 Rabbit Polyclonal Antibody (Product # 720367 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining of neuronal somata and terminals is completely neutralized by preincubation of the antibody with its immunizing peptide.