Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- bs-0237R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-0237R, RRID:AB_10859172
- Product name
- 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic peptide derived from human 14-3-3
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
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Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- L1 mouse brain lysates L2 mouse kidney lysates probed with Anti 14-3-3(Alpha/Beta/Gamma/Delta/Epsilon) Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 31kD. Observed band size: 31kD.
- Sample type
- Mouse
- Other comments
- Brain
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-14-3-3 Polyclonal Antibody (bs-0237R, Unconjugated) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) at 1:200 for 40 minutes at 37°C
- Sample type
- Human
- Other comments
- Colon