GTX113297

antibody from GeneTex

Targeting: YWHAB YWHAA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX113297

Provider

GeneTex

Proper citation

GeneTex Cat#GTX113297, RRID:AB_1952627

Product name

14-3-3 beta antibody [N1C3]

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE GTX113297 diluted at 1:10000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE GTX113297 diluted at 1:10000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] (GTX113297) diluted at 1:5000 and competitor's antibody (sc-628) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta (GTX113297) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer, using 14-3-3 beta(GTX113297) antibody at 1:500 dilution.