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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-38663 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 14-3-3 beta Monoclonal Antibody (5B5G10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody has been tested in indirect ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5B5G10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of 14-3-3 alpha/beta using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) in HeLa (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) against human YWHAB (AA: 1-246) recombinant protein. (Expected MW is 54 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of 14-3-3 alpha/beta using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) in HEK293 (1) and YWHAB (AA: 1-246)-human IgG-Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of 14-3-3 alpha/beta using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) in HeLa (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) against human YWHAB (AA: 1-246) recombinant protein. (Expected MW is 54 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of 14-3-3 alpha/beta using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) in HEK293 (1) and YWHAB (AA: 1-246)-human IgG-Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of 14-3-3 alpha/beta in GC-7901 cells using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of 14-3-3 alpha/beta in HeLa cells using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of 14-3-3 alpha/beta in HepG2 cells using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of 14-3-3 alpha/beta in paraffin-embedded rectum cancer tissues using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of 14-3-3 alpha/beta in paraffin-embedded endometrial cancer tissues using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of 14-3-3 alpha/beta in HeLa cells using 14-3-3 alpha/beta Monoclonal Antibody (Product # MA5-38663) (green) and negative control (red).
