Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29689 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 14-3-3 beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, YWHAB shRNA-transfected 293T. Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (89%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 beta using 30 µg of Raji lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a 14-3-3 beta polyclonal antibody (Product # PA5-29689) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of A-431 (Lane 1), A-375 (Lane 2), U-87 MG (Lane 3), MCF7 (Lane 4), HeLa (Lane 5) and HCT 116 (Lane 6). The blot was probed with Anti-14-3-3 beta antibody (Product # PA5-29689, 1µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 28 kDa band corresponding to 14-3-3 beta was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using 14-3-3 beta Polyclonal Antibody (Product # PA5-29689). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. 14-3-3 beta Polyclonal Antibody (Product # PA5-29689) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of A-431 (Lane 1), A-375 (Lane 2), U-87 MG (Lane 3), MCF7 (Lane 4), HeLa (Lane 5) and HCT 116 (Lane 6). The blot was probed with Anti-14-3-3 beta antibody (Product # PA5-29689, 1µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 28 kDa band corresponding to 14-3-3 beta was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 14-3-3 beta in paraformaldehyde-fixed HeLa cells using a 14-3-3 beta polyclonal antibody (Product # PA5-29689) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using 14-3-3 beta (Product # PA5-29689) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.