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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BML-SA479-0025 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-SA479-0025, RRID:AB_2050492
- Product name
- [pSer185]14-3-3, pAb
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Affinity purified phospho-specific antibody.
- Vial size
- 25 µl
Submitted references Increased 14-3-3 phosphorylation observed in Parkinson's disease reduces neuroprotective potential of 14-3-3 proteins.
Slone SR, Lavalley N, McFerrin M, Wang B, Yacoubian TA
Neurobiology of disease 2015 Jul;79:1-13
Neurobiology of disease 2015 Jul;79:1-13
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Western blot analysis performed with BML-SA479 antibody (1:500), secondary anti-sheep antibody (1:2'000), blotted against 2µg each of: 1. Recombinant 14-3-3_ expressed in E.coli 2. Recombinant 14-3-3_ expressed in E.coli 3. Sheep brain homogenate, which contains ~50% ofÊ_ andÊ_ isoforms that are phosphorylated on Ser185.
