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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [3]
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- Product number
- LS-C313483 - Provider product page
- Provider
- LSBio
- Product name
- SFN / Stratifin / 14-3-3 Sigma Antibody (aa140-156) LS-C313483
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Horse, Porcine, Rabbit, Sheep, Simian
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ICC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody. 14-3-3 sigma was detected in immunocytochemical section of human Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-14-3-3 sigma Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ICC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody. 14-3-3 sigma was detected in immunocytochemical section of human Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-14-3-3 sigma Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ICC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody. 14-3-3 sigma was detected in immunocytochemical section of human Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-14-3-3 sigma Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SFN / Stratifin / 14-3-3 Sigma antibody. IHC(P): Human Esophagus Squama Cancer Tissue.
