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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX100289 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100289, RRID:AB_1240391
- Product name
- 14-3-3 sigma antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references TRIM25 is associated with cisplatin resistance in non-small-cell lung carcinoma A549 cell line via downregulation of 14-3-3σ.
Epstein-Barr virus (EBV) Rta-mediated EBV and Kaposi's sarcoma-associated herpesvirus lytic reactivations in 293 cells.
Qin X, Qiu F, Zou Z
Biochemical and biophysical research communications 2017 Nov 4;493(1):568-572
Biochemical and biophysical research communications 2017 Nov 4;493(1):568-572
Epstein-Barr virus (EBV) Rta-mediated EBV and Kaposi's sarcoma-associated herpesvirus lytic reactivations in 293 cells.
Chen YJ, Tsai WH, Chen YL, Ko YC, Chou SP, Chen JY, Lin SF
PloS one 2011 Mar 10;6(3):e17809
PloS one 2011 Mar 10;6(3):e17809
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 sigma antibody detects SFN protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract12% SDS-PAGE14-3-3 sigma antibody (GTX100289) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 sigma antibody detects SFN protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extractB. 30 ?g GL261 whole cell lysate/extract12% SDS-PAGE14-3-3 sigma antibody (GTX100289) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 sigma antibody detects SFN protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract12% SDS-PAGE14-3-3 sigma antibody (GTX100289) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody (GTX100289) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 sigma antibody [C1C3] detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold Methanol for 5 min.Green: 14-3-3 sigma protein stained by 14-3-3 sigma antibody [C1C3] (GTX100289) diluted at 1:500.Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded 59T xenograft, using 14-3-3 sigma(GTX100289) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 sigma antibody detects 14-3-3 sigma protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. 14-3-3 sigma antibody (GTX100289) dilution: 1:500.
