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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-34644 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 14-3-3 sigma Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, 293T, A431, HeLa, HepG2, A375, Neuro2A, GL261, C8D30.
- Concentration
- 0.32 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 14-3-3 sigma was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a 14-3-3 sigma Polyclonal Antibody (Product # PA5-34644) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 sigma Polyclonal Antibody detects SFN protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract. E. 30 µg A375 whole cell lysate/extract.12% SDS-PAGE.14-3-3 sigma Polyclonal Antibody (Product # PA5-34644) dilution: 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 sigma Polyclonal Antibody detects SFN protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract.12% SDS-PAGE.14-3-3 sigma Polyclonal Antibody (Product # PA5-34644) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 sigma Polyclonal Antibody detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 14-3-3 sigma protein stained by 14-3-3 sigma Polyclonal Antibody (Product # PA5-34644) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 sigma Polyclonal Antibody detects 14-3-3 sigma protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin.14-3-3 sigma Polyclonal Antibody (Product # PA5-34644) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using 14-3-3 sigma (Product # PA5-34644) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 sigma Polyclonal Antibody immunoprecipitates SFN protein in IP experiments. IP samples: A431 whole cell extract. A. 40 µg A431 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of SFN protein by 4 µg 14-3-3 sigma Polyclonal Antibody (Product # PA5-34644). 5 % SDS-PAGE. The immunoprecipitated SFN protein was detected by 14-3-3 sigma Polyclonal Antibody (Product # PA5-34644) diluted at 1:500.
