Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-34644 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 14-3-3 sigma Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, 293T, A431, HeLa, HepG2, A375, Neuro2A, GL261, C8D30. Predicted reactivity: Mouse (98%), Pig (96%), Chicken (81%), Sheep (97%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.32 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 sigma Polyclonal Antibody detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: 14-3-3 sigma protein stained by 14-3-3 sigma Polyclonal Antibody (Product # PA5-34644) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 sigma Polyclonal Antibody immunoprecipitates SFN protein in IP experiments. IP samples: A431 whole cell extract. A. 40 µg A431 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of SFN protein by 4 µg 14-3-3 sigma Polyclonal Antibody (Product # PA5-34644). 5 % SDS-PAGE. The immunoprecipitated SFN protein was detected by 14-3-3 sigma Polyclonal Antibody (Product # PA5-34644) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using 14-3-3 sigma (Product # PA5-34644) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 sigma Polyclonal Antibody detects 14-3-3 sigma protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin.14-3-3 sigma Polyclonal Antibody (Product # PA5-34644) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 sigma Polyclonal Antibody immunoprecipitates SFN protein in IP experiments. IP samples: A431 whole cell extract. A. 40 µg A431 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of SFN protein by 4 µg 14-3-3 sigma Polyclonal Antibody (Product # PA5-34644). 5 % SDS-PAGE. The immunoprecipitated SFN protein was detected by 14-3-3 sigma Polyclonal Antibody (Product # PA5-34644) diluted at 1:500.
