H00002810-M02

antibody from Abnova Corporation

Targeting: SFN YWHAS

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002810-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002810-M02, RRID:AB_1111871

Product name

SFN monoclonal antibody (M02), clone 2G2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant SFN.

Antigen sequence

MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEE
LSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSN
EEGSEEKGPEVREYREKAETELQGVCDTVLGLLDS
HLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDK
KRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALN
FSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSE
DSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQE
PQS


Isotype

IgG

Antibody clone number

2G2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SFN monoclonal antibody (M02), clone 2G2. Western Blot analysis of SFN expression in U-2 OS ( Cat # L022V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SFN is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SFN on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol