LS-C742554

antibody from LSBio

Targeting: SFN YWHAS

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C742554

Provider

LSBio

Product name

SFN / Stratifin / 14-3-3 Sigma Antibody (Internal Region, Biotin) LS-C742554

Antibody type

Polyclonal

Description

ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Reactivity

Human, Mouse

Host

Goat

Conjugate

Biotin

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Biotinylated antibody (0.1µg/ml) staining of Mouse Skin lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocke

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Biotinylated antibody (0.1µg/ml) staining of Mouse Skin lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocke