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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [3]
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- Product number
- RQ5791 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ACADSB Antibody / SBCAD
- Antibody type
- Polyclonal
- Description
- This highly specific ACADSB antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry applications with human, mouse and rat, monkey samples.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ACADSB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human A431, 2) human T-47 D, 3) human A549, 4) monkey COS-7, 5) rat liver and 6) mouse liver lysate with ACADSB antibody. Expected molecular weight: ~47 kDa (unprocessed), ~43 kDa (processed).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human MCF7 cells with ACADSB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human ThP-1 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse RAW264.7 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of rat RH-35 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.
