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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-29958 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SBCAD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, ACADSB-transfected 293T.
- Concentration
- 1 mg/mL
Submitted references HDAC inhibitors elicit metabolic reprogramming by targeting super-enhancers in glioblastoma models.
Nguyen TTT, Zhang Y, Shang E, Shu C, Torrini C, Zhao J, Bianchetti E, Mela A, Humala N, Mahajan A, Harmanci AO, Lei Z, Maienschein-Cline M, Quinzii CM, Westhoff MA, Karpel-Massler G, Bruce JN, Canoll P, Siegelin MD
The Journal of clinical investigation 2020 Jul 1;130(7):3699-3716
The Journal of clinical investigation 2020 Jul 1;130(7):3699-3716
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SBCAD was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SBCAD Polyclonal Antibody (Product # PA5-29958) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SBCAD Polyclonal Antibody (Product # PA5-29958). Sample (30 µg of whole cell lysate). Lane A: HepG2. 10% SDS PAGE. SBCAD Polyclonal Antibody (Product # PA5-29958) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SBCAD antibody detects SBCAD protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: SBCAD protein stained by SBCAD antibody (Product # PA5-29958) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SBCAD antibody detects SBCAD protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: SBCAD protein stained by SBCAD antibody (Product # PA5-29958) diluted at 1:5,000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using SBCAD (Product # PA5-29958) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
