Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B10614 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ SERPINB2 / PAI-2 Antibody (Internal) LS-B10614
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Simian
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-PAI-2 antibody - Western Blot. Western blot of affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 ug of HeLa whole cell lysates. The band at ~42 kD (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:5000 dilution of HRP- conjugated Gt-a-Rabbit IgG (LS-C60865) for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers. Personal communication, Luca DAgostino, Temple University, Philadelphia, PA.