Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA319160 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Plasminogen Activator Inhibitor 2 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Plasminogen Activator Inhibitor 2 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SERPINB2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.2 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 ug of HeLa whole cell lysates. The band at ~42 kDa (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at 1:500in 5% BLOTTO in PBS reacted overnight at 4?. The membrane was washed and reacted with a 1:5,000 dilution of HRP- conjugated Gt-a-Rabbit IgG (p/n 611-103-122) for 45 min at RTwith ECL used for detection.
- Validation comment
- WB
