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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- MAB85501 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Serpin B2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Serpin B2 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P05120- Isotype
- IgG
- Antibody clone number
- 930119
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Serpin B2 by Simple WesternTM. Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. Specific bands were detected for Serpin B2 at approximately 45 & 50 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Serpin B2 by Western Blot. Western blot shows lysates of HEK001 human epidermal keratinocyte cell line (untreated) and U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Show Human Serpin B2 Specificity Using Knockout Cell Line. Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Serpin B2 knockout K562 cell line (KO) untreated (-) or treated (+) with 300nM PMA for overnight. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 40 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. HSP60 (Catalog # MAB1800) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
