Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C745329 - Provider product page
- Provider
- LSBio
- Product name
- SERPINB2 / PAI-2 Antibody (Internal) LS-C745329
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Simian
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using the affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 µg of HeLa whole cell lysates. The band at ~42 kDa (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:5,000 dilution of HRP- conjugated Gt-a-Rabbit IgG for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of rabbit anti-Plasminogen Activator 2 antibody. Tissue: heart. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Plasminogen Activator 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Plasminogen Activator II as precipitated red signal with hematoxylin purple nuclear counterstain.