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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-33188 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33188, RRID:AB_2254405
- Product name
- Rabbit Polyclonal SerpinB2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SerpinB2 Antibody [NBP1-33188] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SerpinB2 Antibody [NBP1-33188] - Sample (30 ug of whole cell lysate) JC 10% SDS PAGE; antibody diluted at 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SerpinB2 Antibody [NBP1-33188] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SerpinB2 Antibody [NBP1-33188] - Immunoprecipitation of PAI2 protein from U87-MG whole cell extracts using 5 ug of PAI2 antibody. Western blot analysis was performed using PAI2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-33188] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
