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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-77964 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77964, RRID:AB_11009648
- Product name
- Rabbit Polyclonal SerpinB2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is directed against human PAI-2 protein. Reactivity occurs against human PAI-2 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.2 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SerpinB2 Antibody [NBP1-77964] - 2 in 50 ug of HeLa whole cell lysates. The band at ~42 kDa (arrowhead) corresponds to SerpinB2 . Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4C. The membrane was washed and reacted with a 1:5,000 dilution of HRP- conjugated Gt-a-Rabbit IgG for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SerpinB2 Antibody [NBP1-77964] - Tissue: heart. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-Plasminogen Activator 2 at 10 ug/mL for 1 h at RT. Staining: Plasminogen Activator II as precipitated red signal with hematoxylin purple nuclear counterstain.
