PA5-27857

antibody from Invitrogen Antibodies

Targeting: SERPINB2 HsT1201, PAI2, PLANH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27857

Provider

Invitrogen Antibodies

Product name

SERPINB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, JC, U87-MG. Predicted reactivity: Mouse (80%), Rabbit (86%), Sheep (81%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.39 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PAI2 using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PAI2 polyclonal antibody (Product # PA5-27857) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SERPINB2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SERPINB2 Polyclonal Antibody (Product # PA5-27857) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SERPINB2 Polyclonal Antibody (Product # PA5-27857). Sample (30 µg of whole cell lysate). Lane A: JC. 10% SDS PAGE. SERPINB2 Polyclonal Antibody (Product # PA5-27857) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SERPINB2 Polyclonal Antibody (Product # PA5-27857). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 10% SDS PAGE. SERPINB2 Polyclonal Antibody (Product # PA5-27857) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SERPINB2 Polyclonal Antibody detects PAI2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAI2 protein stained by SERPINB2 Polyclonal Antibody (Product # PA5-27857) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PAI-2 (Product # PA5-27857) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.