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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- CF500733 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DPP4 Monoclonal Antibody (OTI11D7), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI11D7
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP4 (RC209466, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP4. Positive lysates LY419637 (100 µg) and LC419637 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP4 (RC209466, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP4. Positive lysates LY419637 (100 µg) and LC419637 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure from citation: Western blot analysis of protein levels of DPP4/8/9 in 293E cells transfected with pcDNA3.1 (Mock), pcDNA3.1-hDPP8 (DPP8) and pcDNA3.1-hDPP9 (DPP9), the purified recombinant human DPP4 protein (rhDPP4) was used as a positive control for the DPP4 antibody, antibodies targeting human DPP4/8/9 (TA500733/TA504366/TA504040) and beta-actin (TA310155) were purchased from OriGene. View Citation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of DPP4 protein level by using anti-DPP4 antibody in mouse MLE15 cells. View Citation
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-DPP4 mouse monoclonal antibody (TA500733) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DPP4(RC209466).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure from citation: Immunohistochemistry of DPP4 protein level by using anti-DPP4 antibody in lungs harvested from BALB/c mice at day 3. Dilution: 1:800 View Citation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-DPP4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500733, Dilution 1:50)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-DPP4 antibody (TA500733), (Red) compared to a nonspecific negative control antibody (TA50011)(Blue).
