Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1180 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DPPIV/CD26 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse DPPIV is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q53TN1
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4.
Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells.
A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques.
Identification of sialic acid-binding function for the Middle East respiratory syndrome coronavirus spike glycoprotein.
Elevated CD26 Expression by Skin Fibroblasts Distinguishes a Profibrotic Phenotype Involved in Scar Formation Compared to Gingival Fibroblasts.
First-in-human phase 1 of YS110, a monoclonal antibody directed against CD26 in advanced CD26-expressing cancers.
Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling.
Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection.
High secretion of interferons by human plasmacytoid dendritic cells upon recognition of Middle East respiratory syndrome coronavirus.
Generation of a transgenic mouse model of Middle East respiratory syndrome coronavirus infection and disease.
Regulation of pulmonary graft-versus-host disease by IL-26+CD26+CD4 T lymphocytes.
Lineage-specific regulation of epigenetic modifier genes in human liver and brain.
Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus.
Regulation of somatostatin receptor 4-mediated cytostatic effects by CD26 in malignant pleural mesothelioma.
CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion.
In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection.
Distinct gene expression-defined classes of gastrointestinal stromal tumor.
Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function.
Haverkamp AK, Lehmbecker A, Spitzbarth I, Widagdo W, Haagmans BL, Segalés J, Vergara-Alert J, Bensaid A, van den Brand JMA, Osterhaus ADME, Baumgärtner W
Scientific reports 2018 Jun 27;8(1):9778
Scientific reports 2018 Jun 27;8(1):9778
Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells.
Chu H, Chan CM, Zhang X, Wang Y, Yuan S, Zhou J, Au-Yeung RK, Sze KH, Yang D, Shuai H, Hou Y, Li C, Zhao X, Poon VK, Leung SP, Yeung ML, Yan J, Lu G, Jin DY, Gao GF, Chan JF, Yuen KY
The Journal of biological chemistry 2018 Jul 27;293(30):11709-11726
The Journal of biological chemistry 2018 Jul 27;293(30):11709-11726
A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques.
Cockrell AS, Johnson JC, Moore IN, Liu DX, Bock KW, Douglas MG, Graham RL, Solomon J, Torzewski L, Bartos C, Hart R, Baric RS, Johnson RF
Scientific reports 2018 Jul 16;8(1):10727
Scientific reports 2018 Jul 16;8(1):10727
Identification of sialic acid-binding function for the Middle East respiratory syndrome coronavirus spike glycoprotein.
Li W, Hulswit RJG, Widjaja I, Raj VS, McBride R, Peng W, Widagdo W, Tortorici MA, van Dieren B, Lang Y, van Lent JWM, Paulson JC, de Haan CAM, de Groot RJ, van Kuppeveld FJM, Haagmans BL, Bosch BJ
Proceedings of the National Academy of Sciences of the United States of America 2017 Oct 3;114(40):E8508-E8517
Proceedings of the National Academy of Sciences of the United States of America 2017 Oct 3;114(40):E8508-E8517
Elevated CD26 Expression by Skin Fibroblasts Distinguishes a Profibrotic Phenotype Involved in Scar Formation Compared to Gingival Fibroblasts.
Mah W, Jiang G, Olver D, Gallant-Behm C, Wiebe C, Hart DA, Koivisto L, Larjava H, Häkkinen L
The American journal of pathology 2017 Aug;187(8):1717-1735
The American journal of pathology 2017 Aug;187(8):1717-1735
First-in-human phase 1 of YS110, a monoclonal antibody directed against CD26 in advanced CD26-expressing cancers.
Angevin E, Isambert N, Trillet-Lenoir V, You B, Alexandre J, Zalcman G, Vielh P, Farace F, Valleix F, Podoll T, Kuramochi Y, Miyashita I, Hosono O, Dang NH, Ohnuma K, Yamada T, Kaneko Y, Morimoto C
British journal of cancer 2017 Apr 25;116(9):1126-1134
British journal of cancer 2017 Apr 25;116(9):1126-1134
Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling.
Lessard J, Pelletier M, Biertho L, Biron S, Marceau S, Hould FS, Lebel S, Moustarah F, Lescelleur O, Marceau P, Tchernof A
PloS one 2015;10(3):e0122065
PloS one 2015;10(3):e0122065
Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection.
Peck KM, Cockrell AS, Yount BL, Scobey T, Baric RS, Heise MT
Journal of virology 2015 Apr;89(8):4696-9
Journal of virology 2015 Apr;89(8):4696-9
High secretion of interferons by human plasmacytoid dendritic cells upon recognition of Middle East respiratory syndrome coronavirus.
Scheuplein VA, Seifried J, Malczyk AH, Miller L, Höcker L, Vergara-Alert J, Dolnik O, Zielecki F, Becker B, Spreitzer I, König R, Becker S, Waibler Z, Mühlebach MD
Journal of virology 2015 Apr;89(7):3859-69
Journal of virology 2015 Apr;89(7):3859-69
Generation of a transgenic mouse model of Middle East respiratory syndrome coronavirus infection and disease.
Agrawal AS, Garron T, Tao X, Peng BH, Wakamiya M, Chan TS, Couch RB, Tseng CT
Journal of virology 2015 Apr;89(7):3659-70
Journal of virology 2015 Apr;89(7):3659-70
Regulation of pulmonary graft-versus-host disease by IL-26+CD26+CD4 T lymphocytes.
Ohnuma K, Hatano R, Aune TM, Otsuka H, Iwata S, Dang NH, Yamada T, Morimoto C
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3697-712
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3697-712
Lineage-specific regulation of epigenetic modifier genes in human liver and brain.
Weng MK, Natarajan K, Scholz D, Ivanova VN, Sachinidis A, Hengstler JG, Waldmann T, Leist M
PloS one 2014;9(7):e102035
PloS one 2014;9(7):e102035
Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus.
Raj VS, Smits SL, Provacia LB, van den Brand JM, Wiersma L, Ouwendijk WJ, Bestebroer TM, Spronken MI, van Amerongen G, Rottier PJ, Fouchier RA, Bosch BJ, Osterhaus AD, Haagmans BL
Journal of virology 2014 Feb;88(3):1834-8
Journal of virology 2014 Feb;88(3):1834-8
Regulation of somatostatin receptor 4-mediated cytostatic effects by CD26 in malignant pleural mesothelioma.
Yamamoto J, Ohnuma K, Hatano R, Okamoto T, Komiya E, Yamazaki H, Iwata S, Dang NH, Aoe K, Kishimoto T, Yamada T, Morimoto C
British journal of cancer 2014 Apr 29;110(9):2232-45
British journal of cancer 2014 Apr 29;110(9):2232-45
CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion.
Havre PA, Dang LH, Ohnuma K, Iwata S, Morimoto C, Dang NH
BMC cancer 2013 Nov 1;13:517
BMC cancer 2013 Nov 1;13:517
In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection.
Eckerle I, Müller MA, Kallies S, Gotthardt DN, Drosten C
Virology journal 2013 Dec 23;10:359
Virology journal 2013 Dec 23;10:359
Distinct gene expression-defined classes of gastrointestinal stromal tumor.
Yamaguchi U, Nakayama R, Honda K, Ichikawa H, Hasegawa T, Shitashige M, Ono M, Shoji A, Sakuma T, Kuwabara H, Shimada Y, Sasako M, Shimoda T, Kawai A, Hirohashi S, Yamada T
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2008 Sep 1;26(25):4100-8
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2008 Sep 1;26(25):4100-8
Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function.
Shin JW, Jurisic G, Detmar M
Experimental cell research 2008 Oct 1;314(16):3048-56
Experimental cell research 2008 Oct 1;314(16):3048-56
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human DPPIV/CD26 by Simple WesternTM. Simple Western lane view shows lysates of LoVo human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 168 kDa (as indicated) using 2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DPPIV/CD26 in Human PBMCs. DPPIV/CD26 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DPPIV/CD26 in Human Psoriatic Skin. DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.