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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ABIN1580410 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Spectrin alpha Chain, Brain (SPTAN1) antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- affinity purified antibody
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3D7
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term or -20°C long term.
- Handling
- Avoid repeated freezing and thawing.
Submitted references The spectrin repeat: a structural platform for cytoskeletal protein assemblies.
Djinovic-Carugo K, Gautel M, Ylänne J, Young P
FEBS letters 2002 Feb 20;513(1):119-23
FEBS letters 2002 Feb 20;513(1):119-23
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blots of extract of bovine spinal cord probed with ABIN1580410. A prominent band running at 240 kDa represents the intact ?-II spectrin heavy chain. More minor bands below this likely represent in vivo proteolytic fragments of ?-II spectrin.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal image of mixed neuron-glial cultures stained with MCA- 3D7 antibody to ?-II spectrin (green) and counterstained for DNA (blue). The ?-II spectrin antibody stains numerous axonal and dendritic profiles in these cultures, and this image shows an optical section through a group of neuronal cell bodies. ABIN1580410 clearly reveals the submembraneous cytoskeleton. Since ?-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody. This antibody also reveals the submembranous cytoskeleton of the axon. They named these proteins
