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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- A301-248A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPTAN1/Alpha II-spectrin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SPTAN1/Alpha II-Spectrin by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), 293T (T; 50 µg), and mouse NIH3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-SPTAN1/Alpha II-Spectrin antibody A301-248A (lot A301-248A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SPTAN1/Alpha II-Spectrin by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SPTAN1/Alpha II-Spectrin (Cat. No. A301-248A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
