PA5-89187

antibody from Invitrogen Antibodies

Targeting: NR2E1 TLL, TLX, XTLL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89187

Provider

Invitrogen Antibodies

Product name

TLX Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: YFRGHKEENG AAAHFPSAAL PAPAFFTAVT QLEPHGLELA AVSTTPERQT LVSLAQPTPK YPHEVNGTPM YLYEVATESV CESAARLLFM SIKWAKSVPA FSTLSLQDQL MLLEDAWREL FVLGIAQWAI PVDANTLLAV SGMNGDNTDS QKLNKIISEI QALQEVVARF RQLRLDATEF ACLKCIVTFK AVPTHSGSEL RSFRNAAAIA ALQDEAQLTL NSYIHTRYPT QPCRFGKLLL LLPALRSISP STIEEVFFKK TIGNVPITRL LSDMYKSSDI; Positive Samples: HL-60, HepG2; Cellular Location: Nucleus

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.93 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using NR2E1 Polyclonal antibody (Product # PA5-89187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TLX in extracts of various cell lines using TLX Polyclonal Antibody (Product # PA5-89187) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TLX was performed in A549 cells using TLX Polyclonal Antibody (Product # PA5-89187).