15059-1-AP

antibody from Proteintech Group

Targeting: HPRT1 HGPRT, HPRT

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

15059-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15059-1-AP, RRID:AB_10638622

Product name

HPRT1 antibody

Antibody type

Polyclonal

Description

KD/KO validated HPRT1 antibody (Cat. #15059-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

CRISPR/Cas9-mediated generation of human embryonic stem cell sub-lines with HPRT1 gene knockout to model Lesch Nyhan disease.

Boissart C, Chatrousse L, Poullion T, El-Kassar L, Giraud-Triboult K, Benchoua A
Stem cell research 2023 Sep;71:103144

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Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas.

Yin J, Wang X, Ge X, Ding F, Shi Z, Ge Z, Huang G, Zhao N, Chen D, Zhang J, Agnihotri S, Cao Y, Ji J, Lin F, Wang Q, Zhou Q, Wang X, You Y, Lu Z, Qian X
Nature communications 2023 Sep 22;14(1):5913

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SIRT2 inhibition protects against cardiac hypertrophy and ischemic injury.

Yang X, Chang HC, Tatekoshi Y, Mahmoodzadeh A, Balibegloo M, Najafi Z, Wu R, Chen C, Sato T, Shapiro J, Ardehali H
eLife 2023 Sep 20;12

---

Blocking of oestrogen signals improves anti-tumour effect regardless of oestrogen receptor alpha expression in cancer cells.

Kajihara N, Ge Y, Seino KI
British journal of cancer 2023 Oct;129(6):935-946

---

Spatiotemporal Regulation of De Novo and Salvage Purine Synthesis during Brain Development.

Mizukoshi T, Yamada S, Sakakibara SI
eNeuro 2023 Oct;10(10)

---

Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways.

Yasuda T, Ishihara T, Ichimura A, Ishihara N
Cell reports 2023 May 30;42(5):112434

---

Identification of Exosome-Related Genes Associated with Prognosis and Immune Infiltration Features in Head-Neck Squamous Cell Carcinoma.

You Y, Du Z, Xu G, Tian Z, Xiao M, Wang Y
Biomolecules 2023 Jun 7;13(6)

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Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells.

Hsu DJ, Gao J, Yamaguchi N, Pinzaru A, Wu Q, Mandayam N, Liberti M, Heissel S, Alwaseem H, Tavazoie S, Tavazoie SF
Science advances 2023 Jan 6;9(1):eade9120

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SIRT2 inhibition protects against cardiac hypertrophy and heart failure.

Yang X, Chang HC, Tatekoshi Y, Balibegloo M, Wu R, Chen C, Sato T, Shapiro J, Ardehali H
bioRxiv : the preprint server for biology 2023 Jan 25;

---

Neurobehavioral deficits of mice expressing a low level of G127V mutant frataxin.

Fil D, Conley RL, Zuberi AR, Lutz CM, Gemelli T, Napierala M, Napierala JS
Neurobiology of disease 2023 Feb;177:105996

---

Guanosine primes acute myeloid leukemia for differentiation via guanine nucleotide salvage synthesis.

Wang H, He X, Li Z, Jin H, Wang X, Li L
American journal of cancer research 2022;12(1):427-444

---

ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peripartum cardiomyopathy in mice.

Kouzu H, Tatekoshi Y, Chang HC, Shapiro JS, McGee WA, De Jesus A, Ben-Sahra I, Arany Z, Leor J, Chen C, Blackshear PJ, Ardehali H
The Journal of clinical investigation 2022 May 16;132(10)

---

Aging is associated with increased brain iron through cortex-derived hepcidin expression.

Sato T, Shapiro JS, Chang HC, Miller RA, Ardehali H
eLife 2022 Jan 11;11

---

Large artery stiffening and mortality in a rat model of early vascular remodeling induced by intrauterine growth restriction and a high-fat diet.

Mankouski A, Miller TA, Dodson RB, Yu B, Yang Y, Liu J, Machin DR, Donato AJ, McKnight RA, Zinkhan EK
Physiological reports 2022 Dec;10(23):e15518

---

MYC promotes cancer progression by modulating m(6) A modifications to suppress target gene translation.

Wu G, Suo C, Yang Y, Shen S, Sun L, Li ST, Zhou Y, Yang D, Wang Y, Cai Y, Wang N, Zhang H, Yang YG, Cao J, Gao P
EMBO reports 2021 Mar 3;22(3):e51519

---

Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages.

Navarro-Guerrero E, Tay C, Whalley JP, Cowley SA, Davies B, Knight JC, Ebner D
Scientific reports 2021 Feb 19;11(1):4245

---

HPRT promotes proliferation and metastasis in head and neck squamous cell carcinoma through direct interaction with STAT3.

Wang L, Wang Y, Han N, Wang X, Ruan M
Experimental cell research 2021 Feb 1;399(1):112424

---

Mitochondrial damage and senescence phenotype of cells derived from a novel frataxin G127V point mutation mouse model of Friedreich's ataxia.

Fil D, Chacko BK, Conley R, Ouyang X, Zhang J, Darley-Usmar VM, Zuberi AR, Lutz CM, Napierala M, Napierala JS
Disease models & mechanisms 2020 Jul 27;13(7)

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Uteroplacental Insufficiency Impairs Cholesterol Elimination in Adult Female Growth-Restricted Rat Offspring Fed a High-Fat Diet.

Zinkhan EK, Yu B, McKnight R
Reproductive sciences (Thousand Oaks, Calif.) 2019 Sep;26(9):1173-1180

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Impact of diet on the persistence of early vascular remodeling and stiffening induced by intrauterine growth restriction and a maternal high-fat diet.

Miller TA, Dodson RB, Mankouski A, Powers KN, Yang Y, Yu B, Zinkhan EK
American journal of physiology. Heart and circulatory physiology 2019 Aug 1;317(2):H424-H433

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Prenatal Exposure to a Maternal High Fat Diet Increases Hepatic Cholesterol Accumulation in Intrauterine Growth Restricted Rats in Part Through MicroRNA-122 Inhibition of Cyp7a1.

Zinkhan EK, Yu B, Schlegel A
Frontiers in physiology 2018;9:645

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Intrauterine growth restriction combined with a maternal high-fat diet increased adiposity and serum corticosterone levels in adult rat offspring.

Zinkhan EK, Yu B, Callaway CW, McKnight RA
Journal of developmental origins of health and disease 2018 Jun;9(3):315-328

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Short-term Low-Dose mTORC1 Inhibition in Aged Rats Counter-Regulates Age-Related Gene Changes and Blocks Age-Related Kidney Pathology.

Shavlakadze T, Zhu J, Wang S, Zhou W, Morin B, Egerman MA, Fan L, Wang Y, Iartchouk O, Meyer A, Valdez RA, Mannick JB, Klickstein LB, Glass DJ
The journals of gerontology. Series A, Biological sciences and medical sciences 2018 Jun 14;73(7):845-852

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A delayed sperm penetration of cumulus layers by disruption of acrosin gene in rats.

Isotani A, Matsumura T, Ogawa M, Tanaka T, Yamagata K, Ikawa M, Okabe M
Biology of reproduction 2017 Jul 1;97(1):61-68

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Intrauterine growth restriction influences vascular remodeling and stiffening in the weanling rat more than sex or diet.

Dodson RB, Miller TA, Powers K, Yang Y, Yu B, Albertine KH, Zinkhan EK
American journal of physiology. Heart and circulatory physiology 2017 Feb 1;312(2):H250-H264

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Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2.

Yang X, Park SH, Chang HC, Shapiro JS, Vassilopoulos A, Sawicki KT, Chen C, Shang M, Burridge PW, Epting CL, Wilsbacher LD, Jenkitkasemwong S, Knutson M, Gius D, Ardehali H
The Journal of clinical investigation 2017 Apr 3;127(4):1505-1516

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Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol and low-density lipoprotein receptor activity in rats.

Zinkhan EK, Zalla JM, Carpenter JR, Yu B, Yu X, Chan G, Joss-Moore L, Lane RH
Physiological reports 2016 Jul;4(13)

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Generation of Hprt-disrupted rat through mouse←rat ES chimeras.

Isotani A, Yamagata K, Okabe M, Ikawa M
Scientific reports 2016 Apr 11;6:24215

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Combination of intrauterine growth restriction and a high-fat diet impairs cholesterol elimination in rats.

Zinkhan EK, Chin JR, Zalla JM, Yu B, Numpang B, Yu X, Jiang C, Callaway CW, McKnight RA, Joss-Moore L, Lane RH
Pediatric research 2014 Nov;76(5):432-40

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Differential expression of interferon-γ and chemokine genes distinguishes Rasmussen encephalitis from cortical dysplasia and provides evidence for an early Th1 immune response.

Owens GC, Huynh MN, Chang JW, McArthur DL, Hickey MJ, Vinters HV, Mathern GW, Kruse CA
Journal of neuroinflammation 2013 May 2;10:56

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 15059-1-AP(HPRT1 antibody) at dilution of 1:2000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using HPRT1 antibody 15059-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 15059-1-AP(HPRT1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 15059-1-AP(HPRT1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue