Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-37245 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HPRT Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mAb against human HPRT1 (AA: FULL(1-218)) recombinant protein. (Expected MW is 50.5 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mAb against HEK293 (1) and HPRT1 (AA: FULL(1-218))-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: HPRT Antibody (5F11A7) [NBP2-37245] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HPRT Antibody (5F11A7) [NBP2-37245] - Analysis of kidney tissues using HPRT1 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HPRT Antibody (5F11A7) [NBP2-37245] - Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): HPRT Antibody (5F11A7) [NBP2-37245] - An intracellular stain was performed on HeLa cells with HPRT Antibody (5F11A7) NBP2-37245APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.