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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA506987 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA506987, RRID:AB_2624025
- Product name
- HPRT1 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)
- Antibody type
- Monoclonal
- Description
- HPRT1 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HPRT1
- Isotype
- IgG
- Antibody clone number
- OTI2D5
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPRT1 (RC200462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPRT1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-HPRT1 mouse monoclonal antibody (TA506987) at 1:100 dilution.
- Validation comment
- IF
