Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- PA5-76760 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HPRT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HPRT1. Samples were incubated with HPRT1 polyclonal antibody (Product # PA5-76760).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HPRT1. Samples were incubated with HPRT1 polyclonal antibody (Product # PA5-76760).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of HPRT1 was achieved by transfecting A549 with HPRT1 specific siRNAs (Silencer® select Product # s6888, s6889). Western blot analysis (Fig. a) was performed using Whole cell extracts from the HPRT1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HPRT1 Polyclonal Antibody (Product # PA5-76760, 1:4000) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HPRT1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-HPRT1 Polyclonal Antibody(Product # PA5-76760) and a 24kDa band corresponding to HPRT1 was observed across cell lines and tissues tested . Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HT-29 (Lane 2), A549 (Lane 3), PC-3 (Lane 4), MCF7 (Lane 5), Hep G2 (Lane 6), A-431 (Lane 7), MOLT-4 (Lane 8), Raji (Lane 9), Mouse Testis (Lane 10), Mouse Skeletal Muscle (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).a non specific band was observed around 45kDa.