PA5-21342

antibody from Invitrogen Antibodies

Targeting: HPRT1 HGPRT, HPRT

Antibody data

Submit

Validation data

Product number: PA5-21342 - Provider product page
Provider: Invitrogen Antibodies
Product name: HPRT1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, PC-12, Rat2, rat brain. Predicted reactivity: Rat (97%), Zebrafish (92%), Xenopus laevis (90%), Dog (96%), Pig (97%), Rabbit (98%), Chicken (91%), Chimpanzee (99%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

HPRT1 protein structure - PA5-21342 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HPRT1 using 30 µg of IMR32 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a HPRT1 polyclonal antibody (Product # PA5-21342) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HPRT1 was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a HPRT1 Polyclonal Antibody (Product # PA5-21342) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HPRT1 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a HPRT1 Polyclonal Antibody (Product # PA5-21342) at a dilution of 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HPRT1 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a HPRT1 Polyclonal Antibody (Product # PA5-21342) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of HPRT1 was achieved by transfecting PC-3 with HPRT1 specific siRNAs (Silencer® select Product # S6889, S6888). Western blot analysis (Fig. a) was performed using Whole cell extracts from the HPRT1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HPRT1 Polyclonal Antibody (Product # PA5-21342, 1 µg/mL ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HPRT1.Two non specific bands were observed around 60 kDa and 110 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-HPRT1 Polyclonal Antibody (Product # PA5-21342) and a ~24kDa band corresponding to HPRT1 was observed across cell lines and tissues tested . Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HT-29 (Lane 2), A549 (Lane 3), PC-3 (Lane 4), MCF7 (Lane 5), Hep G2 (Lane 6), A-431 (Lane 7), MOLT-4 (Lane 8), Raji (Lane 9) and Mouse Testis (Lane 10), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Two non specific bands were observed around 60 and 110 kDa.